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Intracytoplasmic sperm injection (ICSI) is a laboratory procedure developed to help infertile couples undergoing in‐ vitro fertilization (IVF) due to severe male factor infertility. ICSI involves the insertion of a single sperm directly into the cytoplasm of a mature egg (oocyte) using a microinjection pipette (glass needle). ICSI has been available for more than fifteen years and achieves a high overall fertilization rate. There is now an alternative to the standard ICSI procedure called the PICSI procedure.

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